Fig. 3. Expression of connexion isoforms in Calu-3 cells. (A) RT-qPCR experiments showed expression of different Cx isoforms. Cx43 and Cx45 were remarkably high expressed, followed by Cx26. The results represent the average ± SEM from three experiments with three cell culture passages. (B) Western blots confirmed protein synthesis of Cx26, Cx43 and Cx45. (C) In immunostainings Cx26, Cx43, and Cx45 were also found at cell-cell contact regions. The scale bar represents 20 µm.